Fig. 5. Concentrations of pro-inflammatory (IL-6 and TNF-α) and pro-angiogenic (VEGF and MCP-1) cytokines in the supernatants collected after co-culture of 4T1 cells and RAW 264.7 macrophages. The cells were co-cultured in conditioned media containing 50% non-treated (CM-NT) or PBP-stimulated (CM-PBP) RAW-CM for 72 h. Cell cultures of the following control groups were also performed: RAW 264.7 macrophages alone (RAW), 4T1 cells alone (4T1), cells stimulated only with PBP (PBP) and cells co-cultured in control medium (vehicle). The levels of mediators in original RAW 264.7 macrophage-conditioned media (CM-NT only and CM-PBP only) were also assessed. Data are shown as the mean ± SEM of six independent experiments. Asterisks show significant differences between the vehicle and all other tested supernatants (***P<0.001; **P<0.01; *P<0.05). Hash marks indicate significant differences between CM-NT and CM-PBP or CM-NT only and CM-PBP only, respectively (^{# # #}P<0,001; ^{# #}P<0.01, ^#P<0.05).